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Image Search Results
Journal: The FASEB Journal
Article Title: Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance
doi: 10.1096/fj.201800059R
Figure Lengend Snippet: Characteristics of serum-extracted EVs. A) Comparison of the sizes of EVs extracted from young murine serum by the ExoQuick reagent pretreated with and without using 0.2-μm filters. B) Morphology of EVs from young murine serum used in this project for rejuvenation of inflammaging, photographed by atomic force microscopy (AFM). C, D) Different miRNA expression profiles in heatmap (C) and quantified summary (D) of EVs from young vs. old murine serum, analyzed by murine miRNA microarray with Mus musculus miRBase version-21 arrays that contained 1900 unique mature miRNA probes (miRNA microarray service via LC Sciences).
Article Snippet: Then, 1–3 μg of total RNAs for each sample were used for customized
Techniques: Comparison, Microscopy, Expressing, Microarray
Journal: Sensors (Basel, Switzerland)
Article Title: Absolute Quantitation of Serum Antibody Reactivity Using the Richards Growth Model for Antigen Microspot Titration
doi: 10.3390/s22103962
Figure Lengend Snippet: Simultaneous titration of antigen density and serum antibody. Steps of the technology, starting with microarray fabrication and measurement, through image analysis to curve fitting and visualization of results are shown. Serum dilution is indicated by red drops, antigen density differences are represented by shades of blue circles. Several binding curves are transformed into one by fitting data with generalized logistic curves, yielding two parameters, x i and d, which characterize thermodynamic activity distribution of serum antibodies. Affinity is related to x i , the point of inflection; antibody heterogeneity is related to d, the asymmetry parameter. The slope at infinity “s” of the curve in the lower left corner is given by the equation shown.
Article Snippet: K.P., Z.H. and J.P. are employed by
Techniques: Titration, Microarray, Binding Assay, Transformation Assay, Activity Assay